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DOSEVAULT
TRTClinical Evidence: Limited

Pregnenolone

Pregnenolone is synthesized from cholesterol by CYP11A1 (the mitochondrial side-chain cleavage enzyme) in steroidogenic tissues including adrenal glands, gonads, and brain. It is the obligate precursor for all downstream steroid hormones: it can be converted to progesterone (via 3-beta-HSD), DHEA (via CYP17A1), or serve as substrate for glucocorticoids and mineralocorticoids. In the brain, pregnenolone and its sulfate ester act as neurosteroids, modulating GABA-A and NMDA receptors and influencing memory and cognition in preclinical models.

Molecular Profile
small-molecule
10 AA Sequence
Evidence grade (Limited): Mostly preclinical or low-quality human studies. This grade reflects the overall quality of available research on this compound and does not constitute a clinical recommendation.
CAS
145-13-1
Mol. weight
316.48 g/mol
Onset and duration

Injectable esters release over days to weeks depending on the ester; oral agents act within hours. Hormonal effects accrue over weeks, and dosing is individualized by a clinician.

Storage and reconstitution

Oral capsule or tablet (supplement or compounded); store at controlled room temperature in a dry place, protected from light and moisture.

Clinical Evidence

What the literature says

Pharmacokinetics

Routes
Oral

Citations (2)

  1. WADA 2026 Prohibited List (pregnenolone not listed); USADA substance profileReview
  2. Pregnenolone - PubChem CID 8955Review

Last reviewed: 2026-06-15

Research Guide

How users approach this compound

The following reflects patterns observed in research literature and community protocols. This is not medical advice and does not constitute a clinical recommendation.

Reported Uses

  • Dietary supplement used for general wellbeing; evidence from clinical trials is sparse
  • Investigated in preclinical research for neurosteroid effects on memory and anxiety

Key Research Benefits

  • Serves as the upstream precursor for all major steroid hormone classes (progesterone, DHEA, glucocorticoids, mineralocorticoids, androgens, and estrogens) in normal physiology
  • Direct neuromodulatory (neurosteroid) activity studied in preclinical models

Potential Side Effects

Limited clinical safety data for supplemental use
Potential hormonal effects depending on downstream steroid conversion
Possible steroid-class adverse reactions at higher doses

Considerations and Cautions

  • Hormone-sensitive conditions (downstream conversion to androgens and estrogens is possible)
  • Pregnancy or breastfeeding (steroid precursor activity; safety data lacking)
  • Not FDA-approved; clinical safety profile is not well characterized

Labs to monitor

Educational context only, not a testing mandate. Any bloodwork decisions belong with your clinician.

  • Estradiol (E2): Potential conversion downstream to estrogens warrants monitoring in sensitive populations.
  • LH and FSH: Downstream steroid conversion may affect hypothalamic-pituitary feedback; contextual monitoring.
  • Lipid panel: Steroid precursor activity; prudent baseline monitoring given potential androgenic/estrogenic downstream products.

Dose Calculator

Peptide Reconstitution Calculator

mg

Total amount of peptide in the vial.

mL

Volume of water used to mix.

mcg

Draw to this mark

10 Units

or 0.10 mL on a U-100 syringe

Concentration:2.50 mg/mL
Peptide per Unit:25.00 mcg/unit
*Always double-check your calculations. This tool is for educational purposes only.

Related Tools

Not medical advice

Information on DoseVault is for educational purposes only and is not a substitute for medical advice, diagnosis, or treatment from a qualified healthcare provider.